Updated: Jul 29, 2026
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| Certification Vendor: | ASCP Board of Certification (ASCP BOC) |
| Exam Name: | MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) |
| Exam Number: | ASCP-MLT |
| Available Languages: | English |
| Related Certifications: | MLT(ASCPi) MLS(ASCP) PBT(ASCP) |
| Exam Duration: | 150 minutes |
| Exam Format: | Computer Adaptive Testing (CAT), Multiple-choice questions |
| Passing Score: | 400 (scale 0-999) |
| Exam Price: | $235 USD (standard); $215 USD (reduced for eligible regions) |
| Certificate Validity Period: | 3 years (renewable via CMP) |
| Real Exam Qty: | 100 |
| Recommended Training: | ASCP Recommended Reading List ASCP BOC MLT Content Guideline |
| Exam Registration: | ASCP BOC Official Application Portal Pearson VUE Testing Center Scheduling |
| Sample Questions: | ASCP ASCP-MLT Sample Questions |
| Exam Way: | Proctored computer-based testing at authorized Pearson VUE test centers |
| Pre Condition: | Associate degree or 60 semester hours from accredited institution + completion of NAACLS/ABHES-accredited MLT program OR approved education + clinical experience; multiple eligibility routes available |
| Official Syllabus URL: | https://www.ascp.org/boc/explore-credentials/view-all-credentials/MLT |
| Section | Weight | Objectives |
|---|---|---|
| Blood Banking (Immunohematology) | 15-20% | - Antibody detection and identification - Blood group systems and antigens - Compatibility testing - Transfusion reactions and adverse events - Blood components and storage |
| Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases - Carbohydrates, lipids, proteins, and enzymes |
| Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition - Physical, chemical, and microscopic analysis - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids |
| Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Red blood cell physiology and disorders - Hemostasis and coagulation testing |
| Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies - Infectious disease serology - Immune system principles and components |
| Microbiology | 15-20% | - Mycology, parasitology, and virology - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility |
| Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes - Laboratory safety and regulatory compliance - Quality control and quality assurance |
1. The risk of HbS polymerization is enhanced by a low (acid) pH, a state of dehydration, and increased levels of
2,3-DPG. Increased temperature (above 37°C) also adds to the risk.
Hematology
Which group of conditions INCREASES the risk of HbS polymerization?
A) Alkaline pH, dehydration, increased level of 2,3-DPG
B) Acid pH,dehydration, increased level of 2,3-DPG
C) Acid pH, dehydration, decreased level of 2,3-DPG
D) Alkaline pH,dehydration, decreased level of 2,3-DPG
2. The ApoB/ApoA1 ratio has a positive risk marker (ApoB-100) and a negative risk marker (ApoA1). Risk diminishes as ApoA1 goes up (a negative marker) and risk increases in proportion to ApoB concentrations (a positive risk marker). This 'double-edged sword' provides good risk prediction. The other risk markers in question do not have this dual-advantage.
Which risk marker has both positive and negative cardiovascular risk integrated into its measurement?
A) LpPLA2
B) Oxidized LDL
C) hs-CRP
D) ApoB/ApoA1
3. Darkfield microscopy allows a lower limit of resolution than bright-field microscopy which helps the tiny spirochetes to be seen in patient samples. In darkfield microscpy, there is a dark background where directly transmitted light is excluded by a dark-field condenser allowing only scattered light to be focused on the specimen. With dark-field microscopy bacteria appear luminous against a dark background.
Which of the following microscopic techniques is best suited for direct examination of the infectious agent of syphilis?
A) Light microscopy
B) Phase microscopy
C) Electron microscopy
D) Darkfield microscopy
4. The intended response is Vitamin B12 and folate deficiencies. Each of these conditions lead to a megaloblastic production of the red blood cells in the bone marrow. Since vitamin B12 and folate are needed in order to produce a synchronous development of the nucleus with the cytoplasm in hematologic cells, oval-macrocytosis often occurs if these nutrients are not in adequate supply within the body. This can also affect neutrophils, allowing for the characteristic hypersegmented nucleus.
The photographic field contains several oval-macrocytes and a hypersegmented neutrophil with greater than 5 nuclear segments. Oval macrocytes are most commonly associated with pernicious anemia and malabsorption syndromes leading to vitamin B12 and folic acid deficiencies.
Clinical information relating to chronic infection, aplastic anemia, and other hematologic maligancies provide the context for the presence of the oval macrocyte.
Macrocytic erythrocytes and hypersegmented neutrophils are not present in thalassemias or in Pelger-Huet anomaly (hyposegmented neutrophils).
Conditions suggested by the macrocytes and the neutrophil in the photograph to the right include which of the following?
A) Pelger-Huet anomaly
B) Vitamin B12 deficiency
C) Folate deficiency
D) Thalassemia
5. Hypochromia can best be described as:
A) Immature red blood cells
B) Decrease in Hgb concentration in RBCs
C) Increase in nucleated RBCs
D) Abnormal erythropoiesis
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: D | Question # 3 Answer: D | Question # 4 Answer: B,C | Question # 5 Answer: A |
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